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Literature summary for 1.7.3.3 extracted from
- A new uricase from Bacillus cereus SKIII Characterization, gene identification and genetic improvement (2020), Int. J. Biol. Macromol., 165, 3135-3144 .
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | 64% residual activity at 1 mM | Bacillus cereus |  |
| Co2+ | 77% residual activity at 1 mM | Bacillus cereus | |
| EDTA | 59% residual activity at 1 mM | Bacillus cereus | |
| Hg2+ | 66% residual activity at 1 mM | Bacillus cereus | |
| Na+ | 69% residual activity at 1 mM | Bacillus cereus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 111% activity at 1 mM | Bacillus cereus | |
| Fe2+ | 109% activity at 1 mM | Bacillus cereus | |
| Mg2+ | 123% activity at 1 mM | Bacillus cereus | |
| Mn2+ | 108% activity at 1 mM | Bacillus cereus | |
| Zn2+ | 105% activity at 1 mM | Bacillus cereus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| urate + O2 + H2O | Bacillus cereus | - |
5-hydroxyisourate + H2O2 | - |
? | |
| urate + O2 + H2O | Bacillus cereus SKIII | - |
5-hydroxyisourate + H2O2 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus cereus | - |
- |
- |
| Bacillus cereus SKIII | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate precipitation, DEAE cellulose column chromatography, and Sephadex G-75 gel filtration | Bacillus cereus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| urate + O2 + H2O | - |
Bacillus cereus | 5-hydroxyisourate + H2O2 | - |
? | |
| urate + O2 + H2O | - |
Bacillus cereus SKIII | 5-hydroxyisourate + H2O2 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 34500, SDS-PAGE | Bacillus cereus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Pucl | - |
Bacillus cereus |
| uricase | - |
Bacillus cereus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
- |
Bacillus cereus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | 60 | the enzyme is stable at 35ºC for 1 h and thermally inactivated at 60°C after 1 h. Rising temperature up to 40°C diminishes the enzyme activity to 83% and 80% after 45 and 60 min, respectively | Bacillus cereus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Bacillus cereus |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Bacillus cereus | isoelectric focusing | - |
7.9 |
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Release 2023.1 (January 2023)
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